An analysis on health management requirements of the middle -aged and old residents in urban community and its influencing factors / 预防医学

Objective To investigate middle -aged and old urban community residents 'demand on community health management as well as the influencing factors.Methods A total of 750 residents over 45 years old already incorporated in the community health management system in Jiangdong District of Ningbo were selected to be investigated by self -made questionnaires item by item and face to face.Results A total of 713(95.07%)valid questionnaires were obtained,and 67.32% of the respondents showed that they needed health management.Multinomial classification logistic analysis indicated that people with more chronic diseases,older age,and higher level of health attention as well as health management cognition were more likely to choose health management.The demand percentages of health management service items from high to low in turn were psychological counseling (82.89%),physical examination for the old residents (77.84%),health counseling training (47.69%),doctors'follow -up and evaluation (34.78%)and the family doctor service (19.78%).The male respondents'demand percentages of psychological counseling and health counseling training were 86.35% and 52.23%respectively,higher than 79.79% and 43.62% in females (P <0.05).The female respondents'demand percentage of doctors'follow -up and evaluation was 38.30%,higher than 30.86% in males (P <0.05).The middle -aged and old residents suffering from chronic diseases had higher demand percentages of psychological counseling,physical examination, <br> health counseling training and the family doctor service than those with no chronic diseases (P <0.01).Conclusion The urban elderly residents'demands on community health management were influenced by chronic diseases and age,andhave health management requirements on psychological counseling and physicel examination.The relevant departments should improve community health management service system.

The influence of occupational exposure and smoking on lung ventilation of coke oven workers / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the influence of occupational exposure and smoking on lung ventilation function of coke oven workers.</p><p><b>METHODS</b>Environmental monitoring was performed on the top, side and bottom of some coke ovens. Lung ventilation function test was performed in 234 coke oven workers.</p><p><b>RESULTS</b>The poison concentration in environment had such tendency as it was the highest on the top, then the side, and the lowest at the bottom. The standardized forced expiratory volume in the 1st second (FEV(1), 90.8% +/- 8.6%) and forced expiratory rate (FEV(1)%, 95.4% +/- 12.4%) of those coke oven workers who smoked were significantly lower than those non-smoking workers (100.9% +/- 14.3%, 108.9% +/- 17.6%); among those smoking workers, the partial correlation coefficients between forced vital capacity (FVC) and benzene soluble, benzopyrene, and smoking index were -0.249, -0.187 and -0.368 respectively; and the coefficients between FEV(1) and the three aspects were -0.255, -0.191 and -0.388; and the coefficients between FEV(1)% and them were -0.131, -0.107 and -0.065. Among those non-smoking workers, the coefficients between benzene solubles and FVC, FEV(1) and FEV(1)% were -0.154, -0.052 and -0.176, and between benzopyrene and them were -0.121, -0.037 and -0.159.</p><p><b>CONCLUSION</b>The lung ventilation function of coke oven workers has certain negative correlation with both occupational exposure and smoking.</p>

Glutathione S-transferase M1, T1 genotypes and the risk of mountain sickness / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To explore the relationship between genetic polymorphisms of glutathione S-transferase (GST) M1, T1 and susceptibility to mountain sickness.</p><p><b>METHODS</b>Forty-three soldiers with acute mountain sickness and 80 healthy soldiers matching with sex/age and training under the same condition were divided into case group and control group. A multiple polymerase chain reaction method was used to detect GSTM1 and GSTT1 genes in genomic DNA isolated from peripheral blood cells from both cases and controls.</p><p><b>RESULTS</b>The frequency of the GSTT1 positive genotype was significantly higher in cases (69.8%) than in controls (42.5%) (P = 0.004, OR = 3.12, 95% CI 1.42 approximately 6.86). The frequency of GSTM1 negative genotype was also higher in cases (72.1%) than in controls (52.5%) (P = 0.03, OR = 2.34, 95% CI 1.05 approximately 5.02). Persons with both GSTM1 and GSTT1 negative genotypes had 5-fold more risk than those with GSTT1 negative and GSTM1 positive genotypes in developing mountain sickness (OR = 5.04, 95% CI: 1.00 approximately 25.3).</p><p><b>CONCLUSION</b>Genetic polymorphisms of glutathione S-transferase M1, T1 may be the risk factors in the development of mountain sickness.</p>

Role of heat shock protein 70 expression in DNA damage induced by benzo(a)pyrene / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To explore heat shock protein 70 (HSP70) expression of A549 cells and its role in DNA damage caused by benzo(a)pyrene (BaP).</p><p><b>METHODS</b>Human adenocarcinoma A549 cells were cultured in vitro, exposed by different concentrations of BaP (0, 1.25, 2.50, 5.00, 10.00 micro mol/L) for 6 hours, or 10 micro mol/L of BaP for different time (0, 4, 8, 12, 16, 24 and 48 h). Then HSP70 expression and DNA damage were detected using Western-blot and single cell gel electrophoresis (SCGE) assay respectively, and the relationship between HSP70 expression and DNA damage was further analyzed.</p><p><b>RESULTS</b>The integral optical densities of HSP70 in A549 cells treated with 1.25, 2.50, 5.00 and 10.00 micro mol/L BaP for 6 h (49.63 +/- 1.30, 45.72 +/- 1.03, 40.53 +/- 0.95, 37.50 +/- 1.20 respectively) were lower than that of the control cells (59.43 +/- 1.17) (P < 0.05). When A549 cells were exposed to 10 micro mol/L BaP for 4, 8, 12, 16 h, the integral optical densities of HSP70 were 33.33 +/- 0.80, 29.23 +/- 0.91, 12.51 +/- 0.96, 9.50 +/- 1.25 respectively, and there was an increasing tendency of the expression of HSP70 for 24 - 48 h (20.06 +/- 1.38, 24.51 +/- 1.39), however, all were different from that in control group (56.59 +/- 0.85) (P < 0.05). DNA damage scores in 10(6) A549 cells treated with 2.50, 5.00 and 10.00 micro mol/L BaP for 6 h (23,718 +/- 2,938, 30,128 +/- 2,937, 44,231 +/- 3,846) were significantly higher than that of the control cell (9,615 +/- 1,923) (P < 0.05). When A549 cells were exposed to 10 micro mol/L BaP for 4, 8, 12, 16, 24, 48 h, DNA damage scores (16,667 +/- 4,003, 38,461 +/- 1,924, 5,615 +/- 3,847, 76,282 +/- 2,937, 7,513 +/- 1,110 and 58,975 +/- 9,487) were also higher than that of control group (P < 0.05). There was a negative correlation between DNA damage and the expression of HSP70 when A549 cells were exposed to different concentrations of BaP.</p><p><b>CONCLUSION</b>HSP70 might enhance intracellular defenses against DNA damage induced by BaP.</p>

Effects of mitogen activated protein kinase signal transduction pathways on heat shock protein 70 gene expression in endothelial cells exposed to benzo(a)pryene / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the effects of mitogen activated protein kinase (MAPK) signal transduction pathways on heat shock protein 70 (HSP70) gene expression in endothelial cells exposed to benzo(a)pryene (BaP).</p><p><b>METHODS</b>Porcine aortic endothelial cells were pre-treated or by PD98059 (10 micro mol/L) or SB203580 (20 micro mol/L) for 1 hour, then treated with different concentrations of BaP (0, 0.1, 0.5, 1.0, 5.0 and 10.0 micro mol/L) for 24 hours respectively;Expression levels of three phosphorylated MAPKs [extracellular signal regulated protein kinase (ERK), c-Jun amino-terminal kinase (JNK), and p38] and HSP70 were determined by Western-blot.</p><p><b>RESULTS</b>The three phosphorylated MAPKs expressional levels especially p-ERK1 had different extents of changes with dose-response relationship under BaP exposure. BaP inhibited the expression of HSP70, which significantly decreased in medium and high dose group (>or= 1.0 micro mol/L) but did not decrease in control group (P < 0.05). Although the inhibitor of ERK (PD98059) could partly weaken the inhibited effects of BaP on HSP70 expression, HSP70 expression levels of endothelial cells pre-treated with PD98059 were still significantly lower than that of control cells (P < 0.05).</p><p><b>CONCLUSION</b>ERK1 pathway might play some roles in HSP70 gene expression in endothelial cells exposed to BaP, and other unknown signal pathways might also have some effects on this process.</p>

Relationship between heat stress protein 70 gene polymorphisms and the risk of acute mountain sickness / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the relationship between heat stress proteins 70 (HSPs70) gene polymorphism and the risk of acute mountain sickness.</p><p><b>METHODS</b>Fifty-six soldiers with acute mountain sickness and 173 soldiers without that were chosen as cases and controls. HSP70-1, HSP70-2 genotypes were analyzed by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.</p><p><b>RESULTS</b>The HSP70-1 polymorphism was similar in two groups. The genotype frequency of HSP70-2 B/B in acute mountain sickness group (23.2%) was significantly higher than that in the control (6.9%, P < 0.05, OR = 4.02).</p><p><b>CONCLUSION</b>There is a significantly increased association of HSP70-2 B/B genotype with the risk of acute mountain sickness. Individuals with HSP70-2 B/B genotype may have weaker adaptive ability than those without this genotype under altitude stress. The results contribute to provide scientific bases for finding these individuals in specified occupational people, ensuring their health and enhancing work efficiency.</p>

Relationship of plasma nitric oxide concentration and the activity of nitric oxide synthase with coronary heart disease / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the levels of nitric oxide synthase (NOS) and nitric oxide (NO) in patients with coronary heart disease (CHD), and the factors affecting them.</p><p><b>METHODS</b>Concentrations of NO and the activities of NOS in plasma in 136 patients and 206 controls using the corresponding Kits were measured. The data were analyzed using covariance and multiple linear regression analysis with SAS 8.1.</p><p><b>RESULTS</b>The levels of NO [(217.05 +/- 153.31) micromol/L] and NOS [(14.09 +/- 7.14) U/ml] in patients were significantly higher than that in the healthy controls [(140.69 +/- 90.96) micromol/L, (7.75 +/- 3.79) U/ml, respectively, P < 0.01]. Smoking and drinking were the independent risk factors for NOS, while sex was the independent risk factor for NO (P < 0.01).</p><p><b>CONCLUSIONS</b>The levels of plasma NO and NOS are closely related with coronary heart disease.</p>

The expression of cytochrome P450 1A1 of endothelial cells induced by benzo(a)pyrene / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To explore the effect of benzo(a)pyrene (BaP) on the expression and the activities of cytochrome P450 1A1 (CYP1A1) of porcine aortic endothelial cells.</p><p><b>METHODS</b>Porcine aortic endothelial cells were cultured in vitro, and treated with different concentrations of BaP (0, 0.5, 1.0, 5.0, 10.0 micro mol/L) for 24 hours, CYP1A1 expression was determined by Western blot and immunohistochemistry. At the same time, the ethoxyresorufin-o-deethylase (EROD) activities were measured by spectrofluorometer.</p><p><b>RESULTS</b>By Western blot, the expression of CYP1A1 of control cells was not found, but the expression of CYP1A1 of cells treated with BaP was found; By immunohistochemistry, only part of endothelial cells treated with BaP had positive expression of CYP1A1. The peak activities of EROD induced by BaP was at the concentration of 0.5 - 1.0 micro mol/L.</p><p><b>CONCLUSION</b>BaP could induce part of endothelial cells to synthesize CYP1A1. BaP of 0.5 - 1.0 micro mol/L could induce peak activities of EROD.</p>